Stage de Recherche niveau Master ou Ingénieur
« Chaire SOLVAY 2019 »
Sujet :
Emulsions eau-dans-eau stabilisées par une membrane de perméabilité modulable pour le contrôle de réactions enzymatiques
Profil candidat(e)s :
Niveau M2 recherche ou Ingénieur en Chimie, ou Chimie-Physique
Compétences souhaitées : physico-chimie des colloïdes et polymères.
Laboratoires d’accueil : Institut des Sciences Moléculaires (ISM, CNRS UMR 5255), groupe NanoSystèmes Analytiques (NSysA ; http://nsysa.ism-bordeaux.cnrs.fr/), localisé à l’ENSCBP, Pessac, France.
Encadrants :
– Pr Valérie RAVAINE, groupe NSysA, ISM ; valerie.ravaine@enscbp.fr; tel : 0556846613.
– Dr Stéphane ARBAULT, groupe NSysA, ISM ; stephane.arbault@enscbp.fr; tel : 0556847907
Objectifs du stage :
Ce stage entre dans le cadre d’un nouveau projet au laboratoire, dont le but est d’explorer les propriétés d’assemblages purement aqueux, basés sur des émulsions eau-dans-eau stabilisées par des nanogels stimulables. Ces systèmes représentent des mimes de la membrane de cellules biologiques. Ils auront vocation à encapsuler une enzyme et à contrôler son activité enzymatique via la perméabilité de la membrane polymérique. Au cours de ce stage, financé par la Chaire Solvay de l’Université de Bordeaux, nous nous focaliserons sur la préparation de nouvelles émulsions eau-dans-eau stabilisées par des nanogels pH-sensibles. La structure et la stabilité de ces émulsions seront caractérisées. La glucose oxydase (GOX) sera choisie comme enzyme modèle à encapsuler. Nous étudierons son taux d’encapsulation et son activité dans les gouttes par spectroscopie et microscopie, dans diverses conditions de perméabilité de membrane. Nous étudierons finalement la réaction de la membrane au processus enzymatique, comme un possible rétrocontrôle de la réaction biochimique.
Techniques utilisées :
Synthèse de particules de polymère – Techniques d’émulsification – Microscopies optique, confocale – Diffusion de lumière – Potentiel Zéta – Microscopie électronique – Spectroscopies.
Temporary Postdoctoral position in Biochemistry
Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials (IPREM)
Université de Pau et des Pays de l’Adour (UPPA), Pau, France
https://iprem.univ-pau.fr/fr/index.html
Recently, UPPA was granted in the second Programme d’Investissements d’Avenir I-SITE (Initiatives Science Innovation, Territoires, Economie) with the project Energy Environment Solutions (E2S). To become an attractive international reference in research in these two fields, E2S UPPA has implemented different research tools, including scientific research chairs: ‘Chaires d’Excellence Partenariales’. The proposed position is in the
framework of one of these chairs: MANTA – Marine Materials (https://e2s-uppa.eu/en/research/chairs-ofexcellence/manta-marine-materials.html).
MANTA combines different disciplines like Biotechnology, Ecology, Chemistry and Material Sciences, and, thematically, involves key areas namely marine by-product and resources valorization, marine bio-inspiration and bio-mimetic materials, littoral environment and water, public health and research & innovation. More specifically, the main objective of MANTA is to mimic remarkable phenomena and hierarchical structures observed in the aquatic environment, to design functional and environmentally sustainable (bio)materials based on marine molecules and assess their impact on human health and marine ecosystems. Public and
private institutions/partners fund MANTA namely E2S UPPA, CAPB, RNA, CIDPMEM through a DLAL project, Laboratoires de Biarritz and Lees.
MANTA research team: PI (Susana Fernandes), 3 PhD students, 1 Post-doc
Host laboratory: IPREM Pau and Anglet, France
IPREM is a joint Research Unit CNRS/UPPA (UMR 5254) in France. IPREM has an extensive and highly competitive research program that comprises the development of fundamental knowledge in physicochemistry, analytical chemistry and microbiology, in relation to applications concerning the structure of the living, the management of the environment and the functional properties of different classes of materials.
Duties/Project description:
The position includes research and teaching duties (64h/year). The position will also include international travel to conferences and meetings with partners/collaborators. From a scientific perspective, this position involves biochemical methods to address the problematic of biomolecules extraction, purification and modification. In particular, enzymatic treatments will be used to improve efficiency and selectivity of the extraction and modification of structurally complex marine molecules.
These approaches will allow accessing to bioactive compounds and providing blocks for hemisynthesis of novel valuable products. We will to characterize the ensuing products using different analytical methods, optical and magnetic spectroscopies, mass spectrometry, diffraction methods, imaging and biochemical characterization.
Requirements:
– To be eligible for the research employment, the candidate must hold a PhD degree in Enzymology, Microbial and Enzymatic Engineering, Biochemistry or similar disciplines, and a large part of the applicant PhD project should have been focused on the use of enzymatic tools for polymer functionalization.
– Extensive experience in experimental research in biocatalysis, enzymology and biochemistry. Strong competences in natural polymer field is a plus.
– Extensive experience in HPLC; GC-MS; NMR, HPAEC-PAD, HPSEC, Crystallogenesis, Crystallography, proteinligand interaction analyses, uniform and specific isotopic labelling.
– The ranking will also accord weight to the candidates assessed competence in ability to interact effectively in a multi-disciplinary research environment.
– The applicant must be proficient in spoken and written English.
Additional qualifications:
Importance will also be placed on personal skills. In this case, we place particular weight on the ability to work as part of a team and a positive attitude towards mobility. The applicant will be responsible for a part of a
larger project, and the ability to work independently and to take responsibility will be required.
Application:
A person with a PhD obtained not more than four years before the end of the application period is particularly eligible for the position.
The application should include:
– CV
– Copy of PhD thesis diploma
– A motivation letter describing the applicant’s previous research experience and how it is related to the present position (one, or maximum two pages) is also required.
– Contact details of two references
The application can be written in English.
The application will be evaluated based on the following criteria:
Appropriate education and work/research in related fields. Candidate motivation, knowledge, scientific maturity and curiosity. Emphasis will also be placed on personal skills.
Selection process:
1. Evaluation of the candidates’ application.
2. If selected, the candidate will have 15 min to present her/his CV and project. The presentation will be followed by questions/discussion.
Monthly Salary before taxes: 35 000 € (according to E2S UPPA index)
Starting date: January/February 2020 or as otherwise agreed.
Type of position: Full-time temporary position ending January/February 2021.
For further information about the position, please contact
Susana Fernandes: susana.fernandes@univ-pau.fr
https://iprem.univ-pau.fr/fr/_plugins/mypage/mypage/content/sfernande004.html